Recombinant Human CCL21 was expressed in E. coli cells.
Catalog No. E882-40N
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
E882-40N-20 | 20 ug | $248 | |
E882-40N-100 | 100 ug | $695 | |
E882-40N-1000 | 1 mg | $3850 | |
E882-40N-BULK | BULK | Contact Us |
Overview:
CCL21, which is a member of the family of CC-Chemokines, has a unique 36 (murine) or 37 (human) amino acid carboxyl-terminal extension not seen in other chemokines. CCL21 selectively stimulates the chemotaxis of adult T lymphocytes and is expressed preferentially in lymph node tissue. CCL21 is critical for adult T cell adhesion to high endothelial venules in lymph nodes, a rate-limiting step for T cell trafficking through nodal tissue.
Gene Aliases:
C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, SLC, Small-inducible cytokine A21, SCYA21, Recombinant Human Secondary Lymphoid-Tissue Chemokine (CCL21).
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight:
12.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
Determined by its ability to chemoattract total lymphocyte population using a concentration range of 10-100 ng/mL.
Purity:
Recombinant Human CCL21 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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