Mouse Monoclonal Antibody
Catalog No. S22-61BM
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S22-61BM-100 | 100 ug | $695 | |
S22-61BM-500 | 500 ug | $2050 | |
S22-61BM-BULK | BULK | Contact Us |
Overview:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
References:
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
Specificity:
Binds with the 101-130 aa region of human ASYN, determined using DB and ELISA with corresponding peptides. Detects ~14kDa band corresponding to ASYN. ~40 kDa is a trimer. Does not detect truncated ASYN (aa 1-114).
Cross Reactivity:
Human, Mouse, Rat
Host/Isotype/Clone#:
Mouse / IgG1 / 10H7; Hybridoma
Immunogen:
Mouse alpha synuclein aggregate
Stability:
1yr at –20⁰C from date of shipment. Avoid freeze/thaw cycle.
Sample Data:
Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Alpha Synuclein Antibody, 10H7 (Cat# S22-61BM). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer)
Sample Data:
Immunocytochemistry/immunofluorescence analysis using Mouse Alpha Synuclein Antibody, 10H7 (Cat# S22-61BM). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.
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