Rabbit Polyclonal Antibody
Catalog No. P64-63DR
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Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kDa protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (2). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (3).
1. Leibersperger, H. et al: Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5; 266(22):14778-84.
2. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.
3. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.
Recognizes the PKCδ/θ protein
Western blot human PKCdelta/theta
PKCdelta/theta from other species may also be detectable.
Synthesized peptide derived from C-terminus of human PKCθ protein
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative Western blot with Anti-PKCδ/θ (1:1000) using 15ng of GST-tagged Human PKCα, PKCβ І, PKCβ ІІ, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ and PKCι recombinant proteins.
Representative Western blot with PKCδ/θ(1:1000) using 20μg, 40μg, 60μg of Jurkat cell lysate.
Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028
J Xue et al., Tumour suppressor TRIM33 targets nuclear [beta]-catenin degradation Nature Communications February 2015 10.1038/ncomms7156
E. McAllister Fiona et al., Correlation profiling for determining kinase-substrate relationships Methods March 2013 10.1016/j.ymeth.2013.03.012
M Tandon et al., SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest. PLoS One March 2015 10.1371/journal.pone.0119346
Sletten Torunn et al., Nucleolin Regulates Phosphorylation and Nuclear Export of Fibroblast Growth Factor 1 (FGF1) PLoS One March 2014 10.1371/journal.pone.0090687
Tandon Manuj et al., New Pyrazolopyrimidine Inhibitors of Protein Kinase D as Potent Anticancer Agents for Prostate Cancer Cells PLoS One September 2013 10.1371/journal.pone.0075601
Decornez H?l?ne et al., Design, Selection, and Evaluation of a General Kinase-Focused Library ChemMedChem June 2009 10.1002/cmdc.200900164
Tokugawa Seiji et al., The expression pattern of PKCθ in satellite cells of normal and regenerating muscle in the rat Neuropathology October 2008 10.1111/j.1440-1789.2008.00967.x
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway