Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C22-18G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| C22-18G-05 | 5 ug | $226 | |
| C22-18G-10 | 10 ug | $325 | |
| C22-18G-BULK | BULK | Contact Us |
Overview:
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes (1). Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated (2).
Gene Aliases:
CDK1: CDC2 CDC28A; MGC111195; DKFZp686L20222 CyclinA2: CCN1; CCNA
References:
1. Vantieghem, A. et al: Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. J. Biol. Chem. 2002; 277(40):37718-31.
2. Rigas, A.C. et al: Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer. Oncogene. 2007; 18.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~59 kDa and CyclinA2 ~78 kDa
Ruiz Josue E et al., Meiotic Inactivation of Xenopus Myt1 by CDK/XRINGO, but Not CDK/Cyclin, via Site-Specific Phosphorylation Molecular Cell October 2008 10.1016/j.molcel.2008.08.029
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
T. Terrano David et al., Cyclin-Dependent Kinase 1-Mediated Bcl-xL/Bcl-2 Phosphorylation Acts as a Functional Link Coupling Mitotic Arrest and Apoptosis Molecular and Cellular Biology February 2010 10.1128/MCB.00882-09
Abbruzzese Genevieve et al., GSK3 and Polo-like kinase regulate ADAM13 function during cranial neural crest cell migration Molecular Biology of the Cell September 2014 10.1091/mbc.E14-05-0970
Zhao Yulei et al., Regulation of sensitivity of tumor cells to antitubulin drugs by Cdk1-TAZ signalling Oncotarget May 2015 10.18632/oncotarget.4259
Dachineni Rakesh et al., Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer Int J Oncol. October 2017 10.3892/ijo.2017.4167
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production Cell reports January 2020
et al., Discovery of novel cyclin-dependent kinase (CDK) and histone deacetylase (HDAC) dual inhibitors with potent in vitro and in vivo anticancer activity. European journal of medicinal chemistry October 2020
et al., Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division The Journal of Biological Chemistry July 2022
et al., Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified Oncotarget November 2016
et al., t?Darpp is an elongated monomer that binds calcium and is phosphorylated by cyclin?dependent kinases 1 and 5 FEBS Open Bio August 2017
Cancer, Cell Cycle, Ser/Thr Kinases
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