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SMAD3 Protein

Recombinant full-length human SMAD3 was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. S12-30G


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Catalog No. Pack Size Price (USD)
S12-30G-50 50 ug $435
S12-30G-20 20 ug $215
S12-30G-BULK BULK Contact Us  


Overview:

SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor (1). SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).


Gene Aliases:

MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZP586N0721, DKFZp686J10186


Genbank Number:


References:


1. Inman, G. J. et al: Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~77 kDa



Product Datasheets



RESEARCH AREAS

AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, WNT Signaling


RELATED PRODUCTS


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  p38 delta, Unactive, M38-14G

  SMAD4 Protein, S13-30G

  Anti-SMAD2, S11-63R


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