Rabbit Polyclonal Antibody
Catalog No. S56-63BR
|Catalog No.||Pack Size||Price (USD)|
STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family. It is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper cells is regulated by members of the STAT family. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 betahydroxylase (2).
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.
Recognizes the STAT5 protein
Western blot STAT5 from human cells
STAT5 from other species may also be detectable.
Synthetic peptide corresponding to amino acids 692-696 of human STAT5 (protein accession number P42229)
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot with Anti-STAT5 (1:1000) using 5 ng of human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5 and STAT6.
Representative western blot with Anti-STAT5 (1:1000) using 3 μg, 10 μg and 30 μg of K562 cell lysate.
A. Cumaraswamy Abbarna et al., Nanomolar-Potency Small Molecule Inhibitor of STAT5 Protein ACS Medical Chemistry Letters September 2014 10.1021/ml500165r
Zeidan N et al., Magnetic bead-based electrochemical detection of interaction between epigallocatechin-3-gallate and STAT proteins Analytical Methods March 2015 10.1039/c5ay00169b
Zhanga Xiaolei et al., Orally bioavailable small-molecule inhibitor of transcription factor Stat3 regresses human breast and lung cancer xenografts PNAS January 2012 10.1073/pnas.1121606109
Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway