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Anti-PKC theta

Rabbit Polyclonal Antibody
Catalog No. P74-63R


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Catalog No. Pack Size Price (USD)
P74-63R-100 100 ug $347
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Overview:

Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).

References:


1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fasligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.

2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.


Specificity:

Recognizes the PKCθ protein


Cross Reactivity:

Western blot human PKCtheta

PKCdelta/theta from other species may also be detectable.


Host:

Rabbit


Immunogen:

Synthetic peptide corresponding to amino acids 674-678 of human PKCθ


Purification:

Affinity chromatography


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot with Anti- PKCθ (1:1000) using 20ng of GST –tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ, and PKCι.



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway


RELATED PRODUCTS


  PKC gamma, Active, P66-10G

  PKC delta, Active, P64-10G

  PKC epsilon, Active, P65-10G

  Anti-PKC delta/theta, P64-63DR


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