Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
P74-63R-100	100 ug	$347
P74-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

P74-63R-100

100 ug

$347

P74-63R-BULK

BULK

Overview:

Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (1). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (2). Insulin resistance plays a primary role in the development of type 2 diabetes and may be related to alterations in fat metabolism. PKC-theta is a crucial component mediating fat-induced insulin resistance in skeletal muscle and is a potential therapeutic target for the treatment of type 2 diabetes (2).

References:

1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fasligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.

2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.

Specificity:

Recognizes the PKCθ protein

Cross Reactivity:

Western blot human PKCtheta

PKCdelta/theta from other species may also be detectable.

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 674-678 of human PKCθ

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti- PKCθ (1:1000) using 20ng of GST –tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ, and PKCι.

Product Datasheets

H270-4

Chia Chuang Huai et al., The kinase GLK controls autoimmunity and NF-_KB signaling by activating the kinase PKC-θ in T cells Nature Immunology October 2011 10.1038/ni.2121

Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-_KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028

J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245

Tokugawa Seiji et al., The expression pattern of PKCθ in satellite cells of normal and regenerating muscle in the rat Neuropathology October 2008 10.1111/j.1440-1789.2008.00967.x

Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112

Anti-PKC theta

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