Rabbit Polyclonal Antibody
Catalog No. H36-63R
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HSP90β is a member of the HSP90 family of proteins which are important molecular chaperones involved in signal transduction, cell cycle control, stress management, and folding, degradation, and transport of proteins (1). HSP90 proteins have been found in a variety of organisms suggesting that they are ancient and conserved. HSP90 binds to client proteins (such as steroid receptors, AKT, Bcr-Abl, Apaf-1, survivin, cyclin dependent kinases) and acts as a molecular chaperone. Failure of HSP90 chaperone activity leads to misfolding of client proteins, which leads to ubiquitination and proteasome degradation, and thus deregulation of cellular homeostasis (2).
1. Csermely, P. et al: The 90-kDa molecular chaperone family: structure, function, and clinical applications. Pharmacol Ther. 1998 Aug;79(2):129-68.
2. Georgakis, G.V. et al: Heat-shock protein 90 inhibitors in cancer therapy: 17AAG and beyond. Future Oncol. 2005 Apr;1(2):273-81.
Recognizes the HSP90β protein
Western blot of human and mouse HSP90b
HSP90b; from other species may also be detectable
Synthetic peptide corresponding to amino acids 252-256 of human HSP90β
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot with Anti-HSP90β (1:1000) using 20ng of human recombinant HSP90α and HSP90β.
Representative western blot with Anti-HSP90β (1:1000) using 3 μg, 10 μg, and 30 μg of HeLa cell lysate.
Suzuki Shigeki et al., Extracellular heat shock protein HSP90β secreted by MG63 osteosarcoma cells inhibits activation of latent TGF-β1 Biochemical and Biophysical Research Communications July 2010 10.1016/j.bbrc.2010.06.112
Dolan Maureen et al., Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins Methods Mol Biol. July 2005 10.1007/978-1-61779-433-9_4
Cancer, Cellular Stress, Inflammation