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Anti-MEK1

Mouse Monoclonal Antibody
Catalog No. M02-60M


For enquiry on all Isoform Specific Antibodies email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
M02-60M-25 25 ug $127
M02-60M-100 100 ug $347
M02-60M-BULK BULK Contact Us  


Overview:

MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).

References:


1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.

2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.


Specificity:

Recognizes the MEK1 protein


Cross Reactivity:

Western blot human MEK1

Immunoprecipitation of human MEK1

MEK1 from other species may also be detectable.


Host / Isotype / Clone#:

Mouse, IgG1, 7F1


Immunogen:

Recombinant full length humanMEK1 protein


Purification:

Affinity purified using Protein G


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Western Blot:


Representative western blot with Anti-MEK1 (1:1000) using 20ng of MEK1 and MEK2 recombinant protein.


Sample Immunoprecipitation:


Representative immunoprecipitation with Anti-MEK1 (2 µg) using 20ng of MEK1 and MEK2 recombinant protein.



Product Datasheets



RESEARCH AREAS

Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology


RELATED PRODUCTS


  MEK1 (EE), Active, M02-12G

  MEK1, Unactive, M02-14H

  MEK3 (MAP2K3), Unactive, M04-14G

  MEK6 (MAP2K6), Unactive, M07-14G


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