Rabbit Polyclonal Antibody
Catalog No. P47-63R
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AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
Recognizes the AMPKα1 protein
Western blot human AMPKa1
AMPK1 from other species may also be detectable.
Synthetic peptide corresponding to amino acids 483-487 of human AMPKα
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot with Anti-AMPK1α (1:1000) using 20 ng of human recombinant AMPK1 and AMPK2.
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
W. Zmijewski Jaroslaw et al., Exposure to Hydrogen Peroxide Induces Oxidation and Activation of AMP-activated Protein Kinase Journal of Biological Chemistry October 2010 10.1074/jbc.M110.143685
M. Beauchamp Elspeth et al., Direct Binding of Arsenic Trioxide to AMPK and Generation of Inhibitory Effects on Acute Myeloid Leukemia Precursors Molecular Cancer Therapeutics October 2014 10.1158/1535-7163.MCT-14-0665-T
Frøsig Christian et al., Exercise-induced TBC1D1 Ser237 phosphorylation and 14-3-3 protein binding capacity in human skeletal muscle Journal of Physiology September 2010 10.1113/jphysiol.2010.194811
JD Klein et al., Metformin, an AMPK activator, stimulates the phosphorylation of aquaporin 2 and urea transporter A1 in inner medullary collecting ducts. American Journal of Physiology - Renal Physiology May 2016 10.1152/ajprenal.00102.2016
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases