Mouse Monoclonal Antibody
Catalog No. M34-60M
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JNK2 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation and treatment of cells with TNFα. JNK2 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK2 has been shown to play an important role in disease processes. JNK2 plays a role in ventricular hypertrophy and is thought to be involved in hypertensive cardiac disease (2).
1. Sluss, H K. et al: Signal transduction by tumor necrosis factor mediated by JNK protein kinases. Mol Cell Biol. 1994 Dec;14(12):8376-84.
2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.
Recognizes only the JNK2 protein
Immunoprecipitation of human JNK2
Western blot of human JNK2.
JNK2 from other species may also be detectable.
Host / Isotype / Clone#:
Mouse, IgG2, 2G12
Human recombinant full length JNK2 protein
Affinity purified using Protein G
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative immunoprecipitation (2μL of anti-JNK2) using 20ng of GST-JNK2 recombinant protein.
Sample Western Blot:
Representative western blot (1:1000) using 20ng of GST-JNK1, GST-JNK2, and GST-JNK3 recombinant protein.
Meter M Van et al., JNK Phosphorylates SIRT6 to Stimulate DNA Double-Strand Break Repair in Response to Oxidative Stress by Recruiting PARP1 to DNA Breaks. Cell Reports September 2016 10.1016/j.celrep.2016.08.006
Hong Park Sun et al., IRAK4 as a Molecular Target in the Amelioration of Innate Immunity?Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid Journal of Immunology November 2014 10.4049/jimmunol.1402101
Selvaraja Nagarathinam et al., Extracellular Signal-Regulated Kinase Signaling Regulates the Opposing Roles of JUN Family Transcription Factors at ETS/AP-1 Sites and in Cell Migration Molecular and Cellular Biology January 2015 10.1128/MCB.00982-14
Ines Cervigni Romina et al., JNK2 controls fragmentation of the Golgi complex and G2/M transition trough phosphorylation of GRASP65 Journal of Cell Science June 2015 10.1242/jcs.164871
Prakasam A et al., JNK1/2 regulate Bid by direct phosphorylation at Thr59 in response to ALDH1L1 Cell Death & Disease July 2014 10.1038/cddis.2014.316
Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, NfkB Pathway