Home    >   Isoform Specific Antibodies

Anti-JNK2

Mouse Monoclonal Antibody
Catalog No. M34-60M


For enquiry on all Isoform Specific Antibodies email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
M34-60M-25 25 ug $127
M34-60M-100 100 ug $347
M34-60M-BULK BULK Contact Us  


Overview:

JNK2 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation and treatment of cells with TNFα. JNK2 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK2 has been shown to play an important role in disease processes. JNK2 plays a role in ventricular hypertrophy and is thought to be involved in hypertensive cardiac disease (2).

References:


1. Sluss, H K. et al: Signal transduction by tumor necrosis factor mediated by JNK protein kinases. Mol Cell Biol. 1994 Dec;14(12):8376-84.

2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.


Specificity:

Recognizes only the JNK2 protein


Cross Reactivity:

Immunoprecipitation of human JNK2

Western blot of human JNK2.

JNK2 from other species may also be detectable.


Host / Isotype / Clone#:

Mouse, IgG2, 2G12


Immunogen:

Human recombinant full length JNK2 protein


Purification:

Affinity purified using Protein G


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Immunoprecipitation:


Representative immunoprecipitation (2μL of anti-JNK2) using 20ng of GST-JNK2 recombinant protein.


Sample Western Blot:


Representative western blot (1:1000) using 20ng of GST-JNK1, GST-JNK2, and GST-JNK3 recombinant protein.



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, NfkB Pathway


RELATED PRODUCTS


  JNK2, Unactive, M34-14G

  Anti-JNK1, M33-63R

  JNK2, Active, M34-10BG

  JNK3, Active, M35-10BG


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox