Human recombinant HDAC3 (full-length)/NCOR2 (237-489) complex was expressed by baculovirus in Sf9 insect cells using a C-terminal GST-tag for HDAC3 and N-terminal GST-tag for NCOR2.
Catalog No. H85-38G
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HDAC3 or Histone deacetylase 3 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC3 may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. Catalytic domain of HDAC4 can interact with HDAC3 via the transcriptional corepressor NCOR2 (1). RELA is a nonhistone substrate of HDAC3 and IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes (2).
HDAC3: HD3; RPD3; RPD3-2 / NCOR2: CTG26; N-CoR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC1
1. Chen, L. et. al: Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001.
2. Fischle, W. et al: Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR. Molec. Cell 9: 45-57, 2002.
Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
HDAC3 ~83 kDa and NCOR2 ~60 kDa
Duan Wenwen et al., Design, Synthesis and Antitumor Evaluation of Novel Histone Deacetylase (HDAC) Inhibitors Equipped with Phenylsulfonylfuroxan Module as Nitric Oxide (NO) Donor Journal of Medicinal Chemistry May 2015 10.1021/acs.jmedchem.5b00317
Hou Xuben et al., Enhancing the Sensitivity of Pharmacophore-Based Virtual Screening by Incorporating Customized ZBG Features: A Case Study Using Histone Deacetylase 8 Journal of Chemical Information and Modeling March 2015 10.1021/ci500762z
Frost Danielle et al., β-Carboline Compounds, Including Harmine, Inhibit DYRK1A and Tau Phosphorylation at Multiple Alzheimer's Disease-Related Sites PLoS One May 2011 10.1371/journal.pone.0019264
Cancer, Cell Cycle, Inflammation