Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
R42-363BR-100	100 ug	$595
R42-363BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

R42-363BR-100

100 ug

$595

R42-363BR-BULK

BULK

Overview:

RHEB is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in the regulation of growth and cell cycle progression. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form. Drosophila RHEB is a direct target of Tsc2 GTPase-activating protein (GAP) activity both in vivo and in vitro. Point mutations in the Drosophila RHEB gene inhibits cell growth while overexpression promotes cell growth. RHEB functions downstream of Tsc1 and Tsc2 in the mTOR signaling pathway.

References:

1. Stocker, H. et al: Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nature Cell Biol. 5: 559-565, 2003.

2. Zhang, Y. et al: Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins. Nature Cell Biol. 5: 578-581, 2003.

Specificity:

Recognizes the RHEB protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

RHEB antibody was raised against a 15 amino acid synthetic peptide from the middle region of human RHEB

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of RHEB in mouse brain cell lysate with RHEB antibody at (A) 1, (B) 2, and (C) 4 ug/ml.