Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
S12-63R-100	100 ug	$595
S12-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

S12-63R-100

100 ug

$595

S12-63R-BULK

BULK

Overview:

SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm (1). Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer (2). There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer (3). SIK3 is a novel tumor-associated antigen (TAA).

References:

1. Al-Hakim A.K., et.al: 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 118:5661-5673(2005).

2. Charoenfuprasert S, et.al: Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene. 2011 Aug 18;30(33):3570-84.

3. Sjoeblom T. et.al: The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).

Specificity:

Recognizes the SIK3 protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.