Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
V14-63R-100	100 ug	$270
V14-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

V14-63R-100

100 ug

$270

V14-63R-BULK

BULK

Overview:

VAV is a proto-oncogene that is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. VAV plays a vital role in hematopoiesis and in T-cell and B-cell development as well as in their activation. VAV is the specific binding partner of Nef proteins from HIV-1 (1). Co-expression and binding of these VAV partners initiates profound morphological changes, cytoskeletal rearrangements and JNK/SAPK pathway activation leading to increased levels of viral transcription and replication. VAV is also the guanine nucleotide dissociation inhibitor for Ras.

References:

1. Fackler, O T. et al: Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions. Molec. Cell 3729-739, 1999.

Specificity:

Recognizes the VAV protein

Cross Reactivity:

Western blot VAV from human cells

VAV from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 172-176 of human VAV

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative Immunoprecipitation with 2 μg of Anti-VAV using 500 μg of Jurkat and THP1cell lysates.