Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
R29-63BR-100	100 ug	$270
R29-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

R29-63BR-100

100 ug

$270

R29-63BR-BULK

BULK

Overview:

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses in unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.

References:

1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12: 141-79.

Specificity:

Recognizes the REL A (NFKB/p65) protein

Cross Reactivity:

Western blot human and mouse REL A

REL A from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 527-531 of human REL A

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative Western blot with Anti-REL A (1:1000) using 20 ng human recombinant Rel A.