Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
A10-63R-100	100 ug	$270
A10-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

A10-63R-100

100 ug

$270

A10-63R-BULK

BULK

Overview:

Activating transcription factor (ATF)-2, also designated CRE-BP1, is a member of the ATF/cyclic AMP-responsive element binding protein family of transcription factors. ATF2 has two unique characteristics: it mediates the adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (1). ATF2 is a target of the JNK signal transduction pathway (2). ATF2 is phosphorylated by JNK on two closely spaced threonine residues within the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, p38 MAPK can also phosphorylate ATF2.

References:

1.Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1.J Biol Chem. 1993 Feb 25; 268(6):4259-66.

2.Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science. 1995 Jan 20; 267(5196):389-93.

Specificity:

Recognizes the ATF2 protein

Cross Reactivity:

Western blot human and mouse ATF2

ATF2 from other species may also be detectable.

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 60-64 of human ATF2

Purification:

Affinity Chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative Western blot with Anti-ATF2 (1:1000) using 10ng of GST-tagged human recombinant ATF2.