Mouse Monoclonal Antibody
Catalog No. A03-60BM
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A03-60BM-100 | 100 ug | $300 | |
A03-60BM-BULK | BULK | Contact Us |
Overview:
Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).
References:
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.
Specificity:
Recognizes the β-actin protein
Cross Reactivity:
Western blot B-actin from human and mouse cells
B-actin from other species may also be detectable.
Host / Isotype / Clone#:
Mouse, IgG2b, BA3R
Immunogen:
Slightly modified human β-cytoplasmic actin (2-15aa) peptide
Purification:
Affinity Chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-β-Actin (1:1000) using 3 μg, 10 μg, and 30 μg of 293 cell lysate.
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