Rabbit Polyclonal Antibody
Catalog No. A16-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A16-63R-100 | 100 ug | $270 | |
A16-63R-BULK | BULK | Contact Us |
Overview:
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).
References:
1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
Specificity:
Recognizes the AKT1, AKT2, and AKT3 protein
Cross Reactivity:
Western blot AKT1, AKT2, and AKT3 from human and mouse cells
AKT1, AKT2, and AKT3 from other species may also be detectable.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to amino acids 471-475 of human AKT
Purification:
Affinity chromatography
Stability:
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-AKT (pan) (1:1000) using 20 ng of human recombinant AKT1, AKT2, and AKT3.
Sample Data:
Representative western blot with Anti-AKT (pan) (1:1000) using 30 μg of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.
Zenga in et al., Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt Molecular Biology of the Cell September 2014 10.1091/mbc.E14-01-0004
Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009
Cancer, Cell Cycle, Inflammation, Neurobiology
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