Rabbit Polyclonal Antibody
Catalog No. P11-363R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P11-363R-100 | 100 ug | $675 | |
P11-363R-BULK | BULK | Contact Us |
Overview:
PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro (1). PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H (2).
References:
1. Hasan, S. et al: Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis. Nature 410: 387-391, 2001.
2. Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
Specificity:
Recognizes the human PCNA protein
Cross Reactivity:
Human, Monkey, Dog, Mouse, Rat, Bovine, Chicken, Fish and Xenopus
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to an internal region of human PCNA
Purification:
Immunoaffinity chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot using affinity purified Anti-PCNA antibody (1:1000) shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Load: ~25 ug of lysate per lane.
There are no related publications available for this product.
Cancer, Cell Cycle, ERK/MAPK Pathway, WNT Signaling
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