Specificity:

Recognizes the human CaM Kinase IV protein

Cross Reactivity:

Human, Mouse, Rat, Dog, Bovine, Chimpanzee

Host Isotype / Clone#:

Rabbit, Antiserum

Immunogen:

The antibody was produced against synthesized peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein.

Purification:

Delipidation, defibrination, buffering and clarification.

Stability:

1yr at –20^oC from date of shipment

Sample Data:

Western blot using Anti-CAMK4 (1:1,000) shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 µg of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown).

Sample Data:

Anti-CAMK4 antibody was diluted 1:500 to detect CAMK4 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.