Specificity:

Recognizes the NOTCH 2 protein

Cross Reactivity:

Human

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human NOTCH 2 located near the N-terminal sequence of the cleaved intracellular domain (NICD).

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Anti-NOTCH 2 was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

References :

1. Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW, Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. 2000.

2. Gridley T , Notch signaling and inherited disease syndromes. Hum. Mol. Genet.: 12, Suppl 1, R9-R13. 2003.