PI3K (p110 beta) is a pool of three individual synthetic siRNA duplexes designed to knock-down human PI3K (p110 beta) mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. P28-911
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The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110? catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation (1). In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110? impedes tumorigenesis with a concomitant diminution of AKT phosphorylation (2).
PIK3CB, PI3K, PIK3C1, PI3Kbeta, MGC133043, p110-BETA, DKFZp779K1237
1. Jackson, S. P. et al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005. 2. Jia, S.et al: Essential roles of PI(3)K-p110-beta in cell growth, metabolism and tumorigenesis. Nature 454: 776-779, 2008.
PI3K (p110 beta) siRNAs are designed to specifically knock-down human PI3K (p110 beta) expression
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ?2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
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AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling