TGFB3 is a pool of three individual synthetic siRNA duplexes designed to knock-down human TGFB3 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. T833-911
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TGFB3 or transforming growth factor, beta 3 is a member of the TGFB family of proteins that is a ser/thr protein kinase. TGFB3 is involved in embryogenesis and cell differentiation. TGFB3 signaling activates transcription of the LEF1 gene to induce epithelial mesenchymal transformation during mouse palate development (1). TGFB3 function in normal palate and lung morphogenesis and implicates this cytokine in epithelial-mesenchymal interaction (2).
ARVD; FLJ16571; TGFB3; TGF-b3
1. Nawshad, A. et.al: TGF-beta-3 signaling activates transcription of the LEF1 gene to induce epithelial mesenchymal transformation during mouse palate development. J. Cell Biol. 163: 1291-1301, 2003.
2. Kaartinen, V. et.al: Abnormal lung development and cleft palate in mice lacking TGF-beta-3 indicates defects of epithelial-mesenchymal interaction. Nature Genet. 11: 415-421, 1995.
TGFB3 siRNAs are designed to specifically knock-down human TGFB3 expression.
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
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