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ROR2 siRNA Set I

ROR2 is a pool of three individual synthetic siRNA duplexes designed to knock-down human ROR2 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. R13-911


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Catalog No. Pack Size Price (USD)
R13-911-05 5 nmol $655
R13-911-20 20 nmol $1205
R13-911-50 50 nmol $2085
R13-911-BULK BULK Contact Us  


Overview:

ROR2 is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. ROR2 is selectively expressed in the chondrocytes of all developing cartilage anlagen, where it is essential during initial growth and patterning, as well as subsequently in the proliferating chondrocytes of mature growth plates, where it is required for normal expansion (1). Thus, ROR2 is selectively expressed in and particularly important for the chondrocyte lineage and loss of ROR2 leads to profound skeletal abnormalities (2).


Gene Alisases:

BDB, BDB1, NTRKR2, MGC163394


Genbank Number:


References:


1. DeChiara, T. M. et al: Ror2, encoding a receptor-like tyrosine kinase, is required for cartilage and growth plate development. Nature Genet. 24: 271-274, 2000.

2. Nomi, M. et al : Loss of mRor1 enhances the heart and skeletal abnormalities in mRor2-deficient mice: redundant and pleiotropic functions of mRor1 and mRor2 receptor tyrosine kinases. Molec. Cell. Biol. 21: 8329-8335, 2001.


Specificity:

ROR2 siRNAs are designed to specifically knock-down human ROR2 expression.


Reconstitution Protocol:

Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.


Storage and Stability:

The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.


Format:

Lyophilized powder



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cardiovascular Disease, Invasion/Metastasis, Neurobiology, Receptor Tyrosine Kinases, WNT Signaling



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