KDM5C is a pool of three individual synthetic siRNA duplexes designed to knock-down human KDM5C mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. K425-911B
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KDM5C or lysine (K)-specific demethylase 5C is a member of the SMCY homolog family that encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. KDM5C is mainly expressed in brain and skeletal muscle. KDM5C is involved in the regulation of transcription and chromatin remodelling that cause X-linked mental retardation (1). KDM5C is also essential for spermatogenesis and expression of male-specific minor histocompatibility antigens (2).
JARID1C; DXS1272E; MRXJ; MRXSJ; XE169
1. Jensen, L. R. et.al: Mutations in the JARID1C gene, which is involved in transcriptional regulation and chromatin remodeling, cause X-linked mental retardation. Am. J. Hum. Genet. 76: 227-236, 2005.
2. Agulnik, A. I. et.al: A mouse Y chromosome gene encoded by a region essential for spermatogenesis and expression of male-specific minor histocompatibility antigens. Hum. Molec. Genet. 3: 873-878, 1994.
KDM5C siRNAs are designed to specifically knock-down human KDM5C expression.
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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