EIF2S1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human EIF2S1 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. E23-911
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EIF2S1, also known as eukaryotic translation initiation factor 2, subunit 1 alpha, is a 35kDa protein in which The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP (1). The Ser51 in the mature human EIF2-alpha protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-alpha also plays an essential role in erythropoiesis (2).
EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
1. Ernst, H. et.al: Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2: characterization of the protein and its messenger RNA. J. Biol. Chem. 262: 1206-1212, 1987.
2. Pathak, V. K.et.al: Generation of a mutant form of protein synthesis initiation factor eIF-2 lacking the site of phosphorylation by eIF-2 kinases. Molec. Cell. Biol. 8: 993-995, 1988
EIF2S1 siRNAs are designed to specifically knock-down human EIF2S1 expression.
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Angiogenesis, Cellular Stress, Inflammation, Neurobiology, Ser/Thr Kinases