MLK3 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway (1). MLK3 can directly phosphorylate and activate IkappaB kinase α and β, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MLK3 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling (2). MLK3 is a component of the BRAF/RAF1 complex and is required for integrity of the complex and for activation of ERK by the complex.
MAP3K11, PTK1, SPRK, MLK3, MGC17114, MLK-3
1. Ing, Y. L. et al: MLK-3: identification of a widely-expressed protein kinase bearing an SH3 domain and a leucine zipper-basic region domain. Oncogene 9: 1745-1750, 1994.
2. Chadee, D. N. et al: MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation. Nature Cell Biol. 6: 770-776,2004.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Das S et al., Mixed lineage kinase 3 promotes breast tumorigenesis via phosphorylation and activation of p21-activated kinase 1. Oncogene January 2019 10.1038/s41388-019-0690-0
Natsuo Takeda Armelle et al., Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1 EMBO Journal August 2014 10.15252/embj.201487808
Saleiro D et al., IFN-γ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5 Science Signaling November 2018 10.1126/scisignal.aap9921
Lee Rubin et al., METHODS, COMPOSITIONS AND KITS FOR PROMOTING MOTOR NEURON SURVIVAL AND TREATING AND DIAGNOSING NEURODEGENERATIVE DISORDERS Patent US 20160082015 April 2016
Apoptosis/Autophagy, Cell Cycle, JNK/SAPK Pathway, Ser/Thr Kinases