RFP or red fluorescent protein from sea anemone Entacmaea quadricolor is a member of the green florescent protein family and is extensively used as a tag and reporter protein in molecular and cellular biology. RFP has a molecular weight of approximately 29 kDa and an excitation and emission maxima at 559 and 611 nm (1). RFP fluoresces over a wide pH range (4-10) with high quantum yield. Like other anthozoan fluorescent proteins, RFP forms tetramers at physiological concentrations.
1. Wiedenmann, J . et al: Red fluorescent protein eqFP611 and its genetically engineered dimeric variants. J Biomed Opt. 2005 Jan-Feb;10(1):14003..
Recognizes native and denatured forms of RFP protein.
Western blot RFP and its variants tag-RFP, DeRed, mCherry and mOrange, etc
Other RFP variants may also be detectable.
Host / Isotype / Clone#:
Mouse, IgG1, RF5R
RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot with Anti-RFP (1:1000) using 5 μl, 10 μl and 20 μl of 293 cell lysate transfected with a RFP protein.
Eskelin K et al., Ribosome profiles and riboproteomes of healthy and potato virus A and Agrobacterium -infected Nicotiana benthamiana plants Molecular Plant Pathology October 2018 10.1111/mpp.12764
A Hafr et al., Formation of Potato Virus A-Induced RNA Granules and Viral Translation Are Interrelated Processes Required for Optimal Virus Accumulation. PLoS Pathogens December 2015 10.1371/journal.ppat.1005314