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Anti-CHK2

Mouse Monoclonal Antibody
Catalog No. C48-60M


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Catalog No. Pack Size Price (USD)
C48-60M-25 25 ug $127
C48-60M-100 100 ug $347
C48-60M-BULK BULK Contact Us  


Overview:

CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).

References:


1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7.

2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.


Specificity:

Recognizes the CHK2 protein


Cross Reactivity:

Western blot CHK2 from human and mouse cells

CHK2 from other species may also be detectable.


Host / Isotype / Clone#:

Mouse, IgG1, 7F9


Immunogen:

Human full length recombinant CHK1


Purification:

Affinity Chromatography


Stability:

Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.


Sample Data:

Representative western blot (1:1000) with Anti-CHK2 using 3 µg, 10 µg, 30µg of 293T cell lysate.



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cell Cycle, Ser/Thr Kinases


RELATED PRODUCTS


  CHK2, Active, C48-10G

  Anti-Mouse HRP, G32-62G


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