Home   >   Secondary Antibodies

Anti-Mouse HRP

Goat Polyclonal Antibody
Catalog No. G32-62G


Shop Online



Catalog No. Pack Size Price (USD)
G32-62G-1000 1 ml $171
G32-62G-BULK BULK Contact Us  


Overview:

Affinity purified mouse IgG was used to raise goat antibodies specific to the mouse IgG. The goat immune serum is subsequently affinity purified using immunoglobulins immobilized to agarose. The antibodies are then conjugated to horse radish peroxidase (HRP) by reductive amination in a 2:1 molar ratio. The HRP conjugated secondary antibody can recognize the primary immunoglobulin and give a signal in the presence of a chemiluminescent substrate. The signal can be detected using an electronic imager or autoradiography film (1).

References:


1. Nakane PK, Pierce GB: Enzyme-labeled antibodies: preparation and application for localization of antigens, J Histochem Cytochem 1966, 14:929


Specificity:

Anti-Mouse HRP conjugated secondary antibody recognizes mouse IgG


Cross Reactivity:

Mouse Immunoglobulin G

(Immunoglobulins from other species may also be detectable)


Host / Isotype / Clone#:

Goat Polyclonal


Immunogen:

Affinity purified mouse immunoglobulins


Purification:

Affinity purified with mouse immunoglobulins immobilized on agarose and adsorbed with IgG from human, rabbit, and bovine


Stability:

Store at 4°C for several months, and at -20°C for longer periods; do not add NaN3 as this will inhibit the HRP enzyme. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot with a mouse monoclonal ERK1 antibody and Anti-Mouse HRP(1:2000) using 5 ug, 10 ug, and 20 ug HeLa cell lysate.



Product Datasheets



RELATED PRODUCTS


  Anti-ERK1, M29-60CM

  Anti-GSK3 beta, G09-60M

  Anti-RSK1, R15-63R

  Anti-CHK2, C48-60M


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox