p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
S6K; PS6K; S6K1; STK14A; RPS6KB1; p70-alpha; p70(S6K)-alpha
1. Ferrari, S. et al: S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. Review.
2. Edelmann, HM. Et al: Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Arif A et al., Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection Molecular Cell February 2019 10.1016/j.molcel.2018.11.017
Yi Sang Ah et al., S6K1 Phosphorylation of H2B Mediates EZH2 Trimethylation of H3: A Determinant of Early Adipogenesis Molecular Cell May 2016 10.1016/j.molcel.2016.03.011
Wang Huizhi et al., Convergence of the Mammalian Target of Rapamycin Complex 1- and Glycogen Synthase Kinase 3-βSignaling Pathways Regulates the Innate Inflammatory Response Journal of Immunology May 2011 10.4049/jimmunol.1002513
Xie Hua et al., Discovery of the novel mTOR inhibitor and its antitumor activities in vitro and in vivo Molecular Cancer Therapeutics March 2013 10.1158/1535-7163.MCT-12-1241
Xie Hua et al., Identification of mammalian target of rapamycin as a direct target of fenretinide both in vitro and in vivo Carcinogenesis July 2012 10.1093/carcin/bgs234
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Metabolic Disorder, Ser/Thr Kinases