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UBA1 (UBE1), Active

Full-length recombinant human UBA1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. U201-380G


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Catalog No. Pack Size Price (USD)
U201-380G-20 20 ug $215
U201-380G-50 50 ug $435
U201-380G-BULK BULK Contact Us  


Overview:

Ubiquitin-activating enzyme 1 (UBA1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. UBA1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Posttranslational modification by ubiquitin or ubiquitin-like proteins regulates numerous processes, including cell division, immune responses and embryonic development.


Gene Aliases:

UBE1, CTD-2522E6.1, A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1X


Genbank Number:


References:


1. Mondal, S. et al: A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510: 41-51, 2016

2. Haas, A.L. et al: Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation. J. Biol. Chem. 257(5):2543-2548, 1982

3. McGrath, J.P. et al: UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J. 10(1):227-236, 1991


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

145 kDa



Product Datasheets



Safety Datasheet



RESEARCH AREAS

Cancer, Cell Cycle, Neurobiology


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