Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies
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Product Name Catalog Number Pack Size Price(USD)  
SHH S851-40N-30 30 ug $270 Contact Us
SHH S851-40N-100 100 ug $556 Contact Us
SHH S851-40N-1000 1 mg $3042 Contact Us
SHH S851-40N-BULK BULK Contact Us  

Description: Recombinant Human SHH was expressed in E. coli cells.

Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications : Cell Assay

Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight : 20.1 kDa

Gene Aliases : SHH, HHG-1, Recombinant Human Sonic Hedgehog (SHH)

Reconstitution Protocol : A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Scientific Background : Highly conserved proteins, members of the Hedgehog family are widely represented throughout the animal kingdom. The three known mammalian Hedgehog proteins; Sonic (SHH), Desert (DHH) and Indian (IHH) hedgehog share a high degree of sequence and structural identity. SHH is specifically known to be expressed in fetal intestine, liver, lung and kidney. Recombinant Human SHH represents the N-product (N-terminal domain with Cys24 substituted by an Ile-Val-Ile sequence) which retains all the known signalling capabilities.

Sample Cell Assay Data : Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1­ - 0.4 µg/mL.

Purity : Recombinant Human SHH resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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