Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies
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Product Name Catalog Number Pack Size Price(USD)  
IL33 I893-40N-10 10 ug $160 Contact Us
IL33 I893-40N-1000 1 mg $5473 Contact Us
IL33 I893-40N-BULK BULK Contact Us  

Description: Recombinant Human IL33 was expressed in E. coli cells.

Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications : Cell Assay

Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight : 18.0 kDa

Gene Aliases : IL-33, Nuclear factor from high endothelial venules, Interleukin-1 family member 11, IL1F-11, C9orf26, NF-HEV
Recombinant Human Interleukin-33 (IL33)

Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background : IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells. IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes. Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL- 33 produced a wide spectrum of cytokines and chemokines. In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

Sample Cell Assay Data : The ED(50) was determined by the dose-dependent proliferation of murine D10S cells is ≤ 0.03 ng/mL.

Purity : Recombinant Human IL33 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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