Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 19.5 kDa
Gene Aliases : Zcyto20, Interleukin-28A, Interferon lambda-2
Reconstitution Protocol : Reconstitute in sterile PBS at 100 μg/mL (containing at least 0.1% human or bovine serum albumin)
Scientific Background : Interferon lambda-2, also known as Interleukin 28A, is an antiviral cytokine with immunomodulatory activity and is distantly related to the IL-10 family and type I interferons. IL28A along with IL29 and IL28B, are three closely related cytokines that form a cytokine gene cluster that use a distinct receptor system which induces antiviral defence signals. IL28A interacts with a heterodimeric class II cytokine receptor ligand and leads to the activation of the JAK/STAT signalling pathway, which ultimately leads to IFN-stimulated gene (ISG) and MHC class I antigen expression. It also plays a key role in antiviral defence in Toll-like receptors (TLR) and intestinal epithelia.
Sample Cell Assay Data : The ED50 is 10.0-50.0 ng/mL as determined by an anti-viral assay (using human hepatocellular carcinoma cells infected with EMC virus)
Purity : Recombinant Human IL17D resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250