Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 15.2 kDa
Gene Aliases : Cytokine ML-1
Reconstitution Protocol : Reconstitute in sterile 4mM HCl at 25μg/mL (containing at least 0.1% human or bovine serum albumin)
Scientific Background : Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins.
Sample Cell Assay Data : The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
Purity : Recombinant Human IL17D resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250