Storage, Stability and Shipping : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 17.7 kDa
Gene Aliases : IL-7, Recombinant Human Interleukin-7 (IL7)
Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Scientific Background : IL7 is a secreted cytokine of the hemopoietin family that primarily affects early B and T cells and plays an important role in stimulating the proliferation of lymphoid progenitors. IL7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. Human and murine IL7 share 60% sequence homology at the primary level and known to exhibit cross species activity. Designated CD127, the human IL7 receptor is a strongly glycosylated membrane protein of 76 kDa. Recombinant Human IL7 is a 17.7 kDa, non-glycosylated protein.
Sample Cell Assay Data : The ED50 as determined by dose-dependent stimulation of mouse lymphoma was <0.5ng/ml
Purity : Recombinant Human IL7 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250