Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 7.8 kDa
Gene Aliases : Insulin-like growth factor II, multiplication stimulating activity, MSA, Somatomedin-A, T3M-11-derived growth factor, PP1446
Reconstitution Protocol : Reconstitute in sterile PBS at 100 μg/mL
Scientific Background : Insulin-like growth factor 2 (IGF2) is one of the mitogenic, polypeptide growth factors that belongs to the insulin family, due to its structural and functional similarity to insulin. IGF2 is mainly produced in the liver and several other tissues at certain times. IGF2 expression can be found in many tissues usually with autocrine, paracrine and endocrine functions. IGF2 mainly acts as a growth promoting hormone during gestation, and does so by binding to the tyrosine kinase type I receptor (IGF1R) as well as the IGF2 receptor cation-independent mannose 6-phosphate receptor. Mature IGF2 is highly conserved between human, bovine, and porcine species. Recombinant Human IGF2 is a single, non-glycosylated, polypeptide chain of 7.8 kDa.
Sample Cell Assay Data : The ED50 as determined by dose dependent proliferation of MCF7 cells was <1ng/ml
Purity : Recombinant Human IGF2 resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250