Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 19.7 kDa
Gene Aliases : Interferon alpha-D, IFN-alpha-1/13, LeIF D, IFNA13
Scientific Background : All known subtypes of IFN- alpha show the same antiviral antiparasitic, antiproliferative activities. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation of various non-receptor tyrosine kinases belonging to the Janus kinases.
Sample Cell Assay Data :
Purity : Recombinant Human IFNA1 (IFN-alpha1) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250