Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight : 15.0 kDa
Gene Aliases : GMF-beta, GMFB, Recombinant Human Glia Maturation Factor Beta (GMFB)
Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Scientific Background : GMF-beta is a 17-kDa brain-specific protein that was isolated from bovine brain homogenate as a substance inducing the maturation of normal neurons as well as glial cells, and at first, it was considered to be a neurotrophic factor. The amino acid sequence of GMFB is highly conserved among many species, suggesting that it plays basic roles across many species. The expression of GMF-b is largely limited to the brain, especially the glial cells and some neurons. Schwann cells of the distal segment of the transected nerve express GMF-b, and this induction of GMF-b coincides with the temporal expression of nerve growth factor receptors in the cell.
Sample Cell Assay Data : ED50 not determined.
Purity : Recombinant Human GMFB resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250