Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 15.0 kDa
Gene Aliases : GDNF, Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF
Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Scientific Background : GDNF is a disulfide-linked, homodimeric neurotrophic factor that belongs to the cystine-knot family of growth factors. Natively glycosylated, GDNF signals through a multi-component receptor system that comprises of a RET and one of the four GFRα receptors. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity Recombinant Human GDNF is a non-glycosylated, disulfide-linked homodimer (30 kDa) consisting of 135 amino acids in each subunit.
Sample Cell Assay Data : The ED50 as determined by the proliferation of rat C6 cells was found to be <0.1 ng/mL
Purity : Recombinant Human GDNF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250