Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o- 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight : 19.7 kDa
Gene Aliases : FGF-21, Recombinant Human Fibroblast Growth Factor 21 (FGF21).
Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Scientific Background : FGF21 is a secreted growth factor of the FGF family, classified as an FGF19 subfamily member. Proteins of the FGF family are known to play a central role with respect to both prenatal and postnatal growth and development, and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. In the presence of beta Klotho (protein cofactor), FGF21 signals via the FGFR receptors to stimulate insulin independent glucose uptake by adipocytes.
Sample Cell Assay Data : The bioactivity was determined in a NIH/3T3 cell proliferation assay with an ED50 <1ng/ml.
Purity : Recombinant Human FGF21 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.