Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight : 23.5 kDa
Gene Aliases : FGF-20, Recombinant Human Fibroblast Growth Factor 20 (FGF20).
Reconstitution Protocol : Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Scientific Background : FGF-20 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-20 shares a high degree of homology with FGF-9 and FGF-16. The group of receptors which FGF-20 binds to comprises of FGFR1c, FGFR2c, FGFR3b, FGFR3c and FGFR4. It is shown that FGF-20 is found in a variety of cells, including but limited to, dopaminergic neurons, fibroblasts, keratinocytes and breast epithelium.
Sample Cell Assay Data : Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.
Purity : Recombinant Human FGF20 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.