Storage, Stability and Shipping : The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight : 22.5 kDa
Gene Aliases : FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, Recombinant Human Fibroblast Growth Factor 8 (FGF8).
Reconstitution Protocol : A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Scientific Background : FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B.
Sample Cell Assay Data : The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Purity : Recombinant Human FGF8 resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.