Storage, Stability and Shipping : Recombinant 4-1BB Receptor can be stored in working aliquots at 2o - 8oC for one month, or at -20oC to -70oC for twelve months. Avoid repeated freeze/thaw cycles.
Applications : Cell Assay
Endotoxin Level : <1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight : 18.0 kDa
Gene Aliases : Tumor necrosis factor receptor superfamily member 9, 41BB, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, CD137, Recombinant Human 4-1BB (TNFRSF9)
Reconstitution Protocol : A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Scientific Background : 4-1BB Receptor (CD137) is a member of the TNF receptor superfamily expressed on activated CD4 and CD8 T cells as well as on activated natural killer cells. The binding of 4-1BB Receptor to its TNF family ligand, 4-1BB ligand (4-1BBL) delivers a co-stimulatory signal to resting T cells leading to high level IL2 production independently of CD28 signaling. Recombinant Human 4-1BB Receptor represents the soluble, cysteine-rich TNFR-like extracellular domain of 4-1BB Receptor.
Sample Cell Assay Data : The ED50 as determined by dose dependent proliferation of human megakaryocytic leukemia cell line is <0.5ng/ml.
Purity : Recombinant Human 4-1BB Receptor (TNFRSF9) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250