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KINASES - LOK, Active (S29-11G)

Product Name Catalog Number Pack Size Price(USD)  
LOK, Active S29-11G-05 5 ug $226 Add to Cart
LOK, Active S29-11G-10 10 ug $325 Add to Cart
LOK, Active S29-11G-BULK BULK Contact Us  


Description: Recombinant human LOK (1-348) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Species: Human

Tag: GST tag

Expression System: Sf9 insect cells using baculovirus

Sequence: 1-348

Genbank Number: NM_005990    


Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.



Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.



Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Kinase Assay, Western Blot

Molecular Weight : ~65 kDa

Gene Aliases : STK10, PRO2729

Scientific Background : LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression (1). LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects (2).

References :
1. Walter, S A. et al: Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue. J Biol Chem. 2003 May 16;278(20):18221-8.

2. Tao, L. et al: Opposing roles of serine/threonine kinases MEKK1 and LOK in regulating the CD28 responsive element in T-cells. Biochem J. 2002 Apr 1;363(Pt 1):175-82.

Product Sheets

SDS

Research Areas
Cardiovascular Disease, Ser/Thr Kinases,

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