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LOK, Active

Recombinant human LOK (1-348) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. S29-11G


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Catalog No. Pack Size Price (USD)
S29-11G-05 5 ug $226
S29-11G-10 10 ug $325
S29-11G-BULK BULK Contact Us  


Overview:

LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression (1). LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects (2).


Gene Alisases:

STK10, PRO2729


Genbank Number:


References:


1. Walter, S A. et al: Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue. J Biol Chem. 2003 May 16;278(20):18221-8.

2. Tao, L. et al: Opposing roles of serine/threonine kinases MEKK1 and LOK in regulating the CD28 responsive element in T-cells. Biochem J. 2002 Apr 1;363(Pt 1):175-82.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~65 kDa



Product Datasheets



Safety Datasheet



There are no related publications available for this product.


RESEARCH AREAS

Cardiovascular Disease, Ser/Thr Kinases


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