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RAF1 (EE), Active

Recombinant human RAF1(Y340E-Y341E, 306-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. R01-13G


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Catalog No. Pack Size Price (USD)
R01-13G-05 5 ug $226
R01-13G-10 10 ug $325
R01-13G-BULK BULK Contact Us  


Overview:

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).


Gene Alisases:

NS5; CRAF; Raf-1; c-Raf


Genbank Number:


References:


1. Rapp, U. et al: Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Nat. Acad. Sci. 80: 4218-4222, 1983.

2. Li, P. et al: Raf-1: a kinase currently without a cause but not lacking in effects. Cell 64: 479-482, 1991.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~63 kDa



Product Datasheets



Safety Datasheet



There are no related publications available for this product.


RESEARCH AREAS

Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases


RELATED PRODUCTS


  ERK2, Unactive, M28-14G

  MEK2, Active, M03-10G

  MEK1, Active, M02-10G

  COT, Unactive, M16-15G


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