Home   >   Enzymes   >   Kinases

COT, Active

Recombinant human COT (30-397) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M16-11G


Shop Online



Catalog No. Pack Size Price (USD)
M16-11G-05 5 ug $226
M16-11G-10 10 ug $325
M16-11G-BULK BULK Contact Us  


Overview:

COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-kappaB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner (1). Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels(2).


Gene Alisases:

MAP3K8, EST, ESTF, TPL2, Tpl-2, c-COT, FLJ10486


Genbank Number:


References:


1. Channavajhala, P L. et al: Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling. J. Biol. Chem. 278: 47089-47097, 2003.

2. Rodríguez C. et al: TRAF6 and Src kinase activity regulates Cot activation by IL-1. Cell Signal. 2006 Sep;18(9):1376-85.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~70 kDa



Product Datasheets



Safety Datasheet



There are no related publications available for this product.


RESEARCH AREAS

Cancer, ERK/MAPK Pathway, Inflammation, NfkB Pathway, Ser/Thr Kinases


RELATED PRODUCTS


  ERK1, Active, M29-10U

  ERK1, Unactive, M29-14U

  MEK2, Active, M03-10G

  MEK1, Active, M02-10G


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox