GTPases & ATPases

ATPases and GTPases are two different families of hydrolase enzymes that catalyze the hydrolysis of the third phosphate of ATP and GTP into ADP and GDP respectively. While human ATPases mainly facilitate the transport of cellular metabolites by harnessing the energy released from the ATP hydrolysis, the GTPases conventionally act as “molecular switches” that are essential mediators of signal transduction in the cells. Dephosphorylation of ATP and GTP is an important chemical process in all forms of life, therefore ATPases and GTPases are spread ubiquitously across the plant and the animal kingdom. Mutations in members of these families of enzymes have been observed in many human diseases such as cancer and metabolic disorders. For example, the founding member of the small GTPase Superfamily, Ras, is mutated in about 15% of all human tumors.

SignalChem manufactures high-quality active GTPase and ATPase enzymes such as G protein α subunits, Ras, Rac, and Rho. Our recombinant ATPases/GTPases are perfect candidates to be included in enzyme activity assays and their inhibitor profiling studies. SignalChem's active GTPases are tested exclusively using Promega's GTPase-Glo™ Assay.